Neuroglobin in rat brain neurons

Neuroglobin is a metalloprotein expressed predominantly in nervous system and involved the functioning of cells normal pathological conditions. Despite numerous studies, information on its regional distribution ambiguous. The purpose study to identify patterns features neuroglobin neurons all structures rat brain with quantitative assessment content at cellular level. Material methods . Five outbred male rats kept under standard conditions were used study. Frontal serial sections made after decapitation, fixation, dehydration paraffin-embedding. One section series was stained according Nissl method for identification stereotaxic atlas, second immunohistochemically neuroglobin. 100 studied cytophotometrically, immunoreactivity units optical density ×10 3 (conventional units). Results discussion Four levels identified – low, moderate, high very high. Structures without not found. Among amount contents varies from 140–160 459–479 relative units. low moderate revealed supreme number structures. parts there an increase direction telencephalon diencephalon midbrain pons medulla oblongata. widest range values found telencephalon, In cerebellum largest are demonstrated by interposed nucleus Purkinje pyramid. Conclusion has been spinal cord. depends phylogenetic age: increases anteroposterior direction, paleocortex contain more than neocortex. greater paleocerebellum.